AMOT Polyklonaler Antikörper

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• KD/KO Validated

AMOT Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 24550-1-AP

Synonyme

KIAA1071, Angiomotin

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  WB analysis of HEK-293 using 24550-1-AP

  WB result of AMOT antibody (24550-1-AP, 1:6000) with si-Control and si-AMOT transfected HEK293 cells.

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  WB analysis using 24550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24550-1-AP (AMOT antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24550-1-AP

  IP result of anti-AMOT (IP:24550-1-AP, 4ug; Detection:24550-1-AP 1:1000) with HEK-293 cells lysate 1280 ug.

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  IHC staining of human colon cancer using 24550-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24550-1-AP (AMOT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24550-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24550-1-AP (AMOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 24550-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AMOT antibody (24550-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

24550-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AMOT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMOT fusion protein Ag19784
• Vollständiger Name: angiomotin
• Berechnetes Molekulargewicht: 1084 aa, 118 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 130 kDa
• GenBank-Zugangsnummer: BC130294
• Gene symbol: AMOT
• Gene ID (NCBI): 154796
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin. This antibody recognizes both p130 and p80.

Publikationen

Species	Application	Title
human	WB	Theranostics; The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy.; Authors - Qiang Guo
human	IF	Sci Rep; MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.; Authors - Diala Kantar
human	IP	PLoS One; Regulation of YAP and Wnt signaling by the endosomal protein MAMDC4; Authors - Christopher M Cox
	WB,IHC	Mol Cell Biochem; PSAT1 promotes the progression of colorectal cancer by regulating Hippo-YAP/TAZ-ID1 axis via AMOT; Authors - Minshan Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jonasz Jeremiasz (Verified Customer) (07-30-2024)

The antibody works perfectly in western blot analysis of human cell lines, detecting both isoforms of the AMOT protein at endogenous expression levels and when overexpressed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: HEK293T

AMOT Antibody Western Blot validation (1:4000 dilution) in HEK293T (Cat no:24550-1-AP)