AMPK Alpha 2 Polyklonaler Antikörper

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• KD/KO Validated

AMPK Alpha 2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18167-1-PBS

Synonyme

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  WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

  WB result of AMPK alpha 2 antibody (18167-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha 2 transfected HEK-293 cells. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18167-1-AP (same clone as 18167-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat ovary using 18167-1-AP (same clone as 18167-1-PBS)

  rat ovary tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18167-1-AP (same clone as 18167-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 18167-1-AP (same clone as 18167-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

  IP result of anti-AMPK Alpha 2 (IP:18167-1-AP, 4ug; Detection:18167-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 18167-1-AP (same clone as 18167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 18167-1-AP (same clone as 18167-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 18167-1-AP (same clone as 18167-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using AMPK Alpha 2 antibody (18167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18167-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18167-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AMPK Alpha 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Alpha 2 fusion protein Ag12796
• Vollständiger Name: protein kinase, AMP-activated, alpha 2 catalytic subunit
• Berechnetes Molekulargewicht: 552 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC069680
• Gene symbol: AMPK Alpha 2
• Gene ID (NCBI): 5563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).