AMPK Gamma 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMPK Gamma 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10290-1-PBS

Synonyme

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  WB analysis using 10290-1-AP (same clone as 10290-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10290-1-AP (AMPK Gamma 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10290-1-AP (same clone as 10290-1-PBS)

  WB result of AMPK Gamma 1 antibody (10290-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK Gamma 1 transfected HEK-293 cells. This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 10290-1-AP (same clone as 10290-1-PBS)

  IP result of anti-AMPK Gamma 1 (IP:10290-1-AP, 4ug; Detection:10290-1-AP 1:1000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10290-1-AP (same clone as 10290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10290-1-AP (same clone as 10290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 10290-1-AP (same clone as 10290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 10290-1-AP (same clone as 10290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10290-1-AP (AMPK gamma 1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10290-1-AP (same clone as 10290-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10290-1-AP (AMPK gamma 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10290-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10290-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA AMPK Gamma 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Gamma 1 fusion protein Ag0302
• Vollständiger Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC000358
• Gene symbol: AMPK Gamma 1
• Gene ID (NCBI): 5571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.

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