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AMPK Alpha 1 Monoklonaler Antikörper
AMPK Alpha 1 Monoklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1H8E1
Kat-Nr. : 66536-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, 4T1-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, Jurkat-Zellen, K-562-Zellen, L02-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, THP-1-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 75 publications below |
IHC | See 4 publications below |
FC | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
66536-1-Ig bindet in WB, IHC, CoIP, ELISA AMPK Alpha 1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Maus, Ratte, Ziege |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | AMPK Alpha 1 fusion protein Ag24744 |
Vollständiger Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
Berechnetes Molekulargewicht | 63 kDa |
Beobachtetes Molekulargewicht | 64 kDa |
GenBank-Zugangsnummer | BC012622 |
Gene symbol | AMPK Alpha 1 |
Gene ID (NCBI) | 5562 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for AMPK Alpha 1 antibody 66536-1-Ig | Protokoll herunterladen |
IHC protocol for AMPK Alpha 1 antibody 66536-1-Ig | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
EMBO J Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes | ||
Redox Biol Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner. | ||
Biomed Pharmacother Berberine is a potential alternative for metformin with good regulatory effect on lipids in treating metabolic diseases | ||
J Nanobiotechnology Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles. | ||
J Nanobiotechnology Graphene oxide exacerbates dextran sodium sulfate-induced colitis via ROS/AMPK/p53 signaling to mediate apoptosis. | ||
Food Chem Selective transportation and energy homeostasis regulation of dietary advanced glycation end-products in human intestinal Caco-2 cells. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH YINGJIAN (Verified Customer) (08-15-2025) | high specificity, strong/robust signal, low background, consistent results, reproducible performance, good lot-to-lot consistency
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