AMPK Alpha Rekombinanter Antikörper

AMPK Alpha Rekombinant Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

4N5

Kat-Nr. : 80209-5-RR

Synonyme

AMPK Alpha 1, PRKAA1, AMPK, Acetyl-CoA carboxylase kinase, ACACA kinase

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  WB analysis using 80209-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80209-5-RR (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 80209-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AMPK Alpha antibody (80209-5-RR, Clone: 4N5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80209-5-RR

  Biolayer interferometry (BLl) kinetic assays of 80209-5-RR against Human AMPK alpha were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

80209-5-RR bindet in WB, IF-P, ELISA AMPK Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AMPK Alpha fusion protein Ag1367
• Vollständiger Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC012622
• Gene symbol: AMPK Alpha 1
• Gene ID (NCBI): 5562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing. 80209-5-RR can recognize both AMPK Alpha 1 and AMPK Alpha 2.

Publikationen

Species	Application	Title
mouse	WB	Life Sci; Idebenone improves mitochondrial respiratory activity and attenuates oxidative damage via the SIRT3-SOD2 pathway in a prion disease cell model; Authors - Zhixin Sun