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  • Featured Product
  • KD/KO Validated

AMPK Alpha Monoklonaler Antikörper

AMPK Alpha Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E1

Kat-Nr. : CL488-66536

Synonyme

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated AMPK Alpha Monoclona (CL488-66536)

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated AMPK Alpha Monoclona (CL488-66536)

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

Geprüfte Anwendungen

Erfolgreiche Detektion in IFhumanes Mammakarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

CL488-66536 bindet in IF AMPK Alpha und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen AMPK Alpha fusion protein Ag24744
Vollständiger Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Berechnetes Molekulargewicht 63 kDa
Beobachtetes Molekulargewicht 64 kDa
GenBank-ZugangsnummerBC012622
Gene symbol PRKAA1
Gene ID (NCBI) 5562
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 AMPK Alpha antibody CL488-66536Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Cell Rep

Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex

Authors - Liping Xie
View Article