AMPK Beta 1 Polyklonaler Antikörper

AMPK Beta 1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26907-1-AP

Synonyme

PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1

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  WB analysis using 26907-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK Beta 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26907-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26907-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26907-1-AP (AMPK beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26907-1-AP bindet in WB, ELISA AMPK Beta 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Beta 1 fusion protein Ag25534
• Vollständiger Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC001056
• Gene symbol: PRKAB1
• Gene ID (NCBI): 5564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880).