AMPK Beta 2 Monoklonaler Antikörper

AMPK Beta 2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5C1

Kat-Nr. : 66579-1-Ig

Synonyme

AMPK subunit beta 2, PRKAB2

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  WB analysis using 66579-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66579-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66579-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66579-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66579-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A431-Zellen, A549-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66579-1-Ig bindet in WB, IF/ICC, ELISA AMPK Beta 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AMPK Beta 2 fusion protein Ag5806
• Vollständiger Name: protein kinase, AMP-activated, beta 2 non-catalytic subunit
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC053610
• Gene symbol: AMPK Beta 2
• Gene ID (NCBI): 5565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.