AMT Monoklonaler Antikörper

AMT Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E7D4

Kat-Nr. : 67532-1-PBS

Synonyme

GCST, 3E7D4, aminomethyltransferase, EC:2.1.2.10, GCVT

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  WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67532-1-Ig (same clone as 67532-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 67532-1-Ig (same clone as 67532-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50403-2

  Cytometric bead array standard curve of MP50403-2, AMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67532-3-PBS. Detection antibody: 67532-1-PBS. Standard:Ag26644. Range: 3.125-100 ng/mL.

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  Affinity and Kinetic Characterization of 67532-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67532-1-Ig against Human AMT was performed. The affinity constant is 8.85 nM.

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Geprüfte Anwendungen

Produktinformation

67532-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA AMT und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AMT fusion protein Ag26644
• Vollständiger Name: aminomethyltransferase
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 37-44 kDa
• GenBank-Zugangsnummer: BC007546
• Gene symbol: AMT
• Gene ID (NCBI): 275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.