Amylase Alpha Polyklonaler Antikörper
Amylase Alpha Polyklonal Antikörper für WB, IHC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF-P, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 12540-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maus-Pankreasgewebe |
| Erfolgreiche Detektion in IHC | humanes Pankreaskarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | Ratten-Pankreasgewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 1 publications below |
| IHC | See 6 publications below |
| IF | See 9 publications below |
Produktinformation
12540-1-AP bindet in WB, IHC, IF-P, ELISA Amylase Alpha und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Amylase Alpha fusion protein Ag3260 |
| Vollständiger Name | amylase, alpha 2B (pancreatic) |
| Berechnetes Molekulargewicht | 511 aa, 58 kDa |
| Beobachtetes Molekulargewicht | 58 kDa |
| GenBank-Zugangsnummer | BC011179 |
| Gene symbol | AMY2B |
| Gene ID (NCBI) | 280 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Amylase Alpha antibody 12540-1-AP | Protokoll herunterladen |
| IHC protocol for Amylase Alpha antibody 12540-1-AP | Protokoll herunterladenl |
| IF protocol for Amylase Alpha antibody 12540-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
ACS Nano Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition | ||
Cancer Lett SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer. | ||
Biochim Biophys Acta Mol Basis Dis Pyrazole derivative Z10 ameliorates acute pancreatitis by inhibiting the ERK/Ddt pathway | ||
Int Immunopharmacol Gastrodin ameliorates acute pancreatitis by modulating macrophage inflammation cascade via inhibition the p38/NF-κB pathway | ||
Mol Biol Cell Vacuolization of mucolipidosis type II mouse exocrine gland cells represents accumulation of autolysosomes. | ||
Biomed Pharmacother Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Gabriele (Verified Customer) (07-18-2025) | The antibody works very well both in WB (1:5000, 5% milk) and IF (1:200, 5% BSA) on HEK293T over-expressing human AMY2A 48h post-transfection vs not-treated control
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FH balawant (Verified Customer) (08-06-2022) | This antibody is working great and need 1:20000 dilution.
|
FH Susmita (Verified Customer) (06-13-2022) | This is an excellent antibody for IHC and WB application
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FH Iram (Verified Customer) (03-11-2022) | Very good antibody for western
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