Amylase Alpha Polyklonaler Antikörper

Amylase Alpha Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 12540-1-AP

Synonyme

AMY2B, Amylase, alpha, 1,4-alpha-D-glucan glucanohydrolase 2B, Alpha amylase, Alpha amylase 2B



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaus-Pankreasgewebe
Erfolgreiche Detektion in IHChumanes Pankreaskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PRatten-Pankreasgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:20000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12540-1-AP bindet in WB, IHC, IF-P, ELISA Amylase Alpha und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Amylase Alpha fusion protein Ag3260
Vollständiger Name amylase, alpha 2B (pancreatic)
Berechnetes Molekulargewicht 511 aa, 58 kDa
Beobachtetes Molekulargewicht 58 kDa
GenBank-ZugangsnummerBC011179
Gene symbol AMY2B
Gene ID (NCBI) 280
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Amylase Alpha antibody 12540-1-APProtokoll herunterladen
IHC protocol for Amylase Alpha antibody 12540-1-APProtokoll herunterladenl
IF protocol for Amylase Alpha antibody 12540-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

ACS Nano

Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition

Authors - Yanan Wang
mouseIF

Cancer Lett

SULF2 enhances GDF15-SMAD axis to facilitate the initiation and progression of pancreatic cancer.

Authors - Ruizhe He

Biochim Biophys Acta Mol Basis Dis

Pyrazole derivative Z10 ameliorates acute pancreatitis by inhibiting the ERK/Ddt pathway

Authors - Wenying Zeng
mouseWB,IHC

Int Immunopharmacol

Gastrodin ameliorates acute pancreatitis by modulating macrophage inflammation cascade via inhibition the p38/NF-κB pathway

Authors - Yalan Jiang
mouseIHC

Mol Biol Cell

Vacuolization of mucolipidosis type II mouse exocrine gland cells represents accumulation of autolysosomes.

Authors - Boonen Marielle M
mouseIF

Biomed Pharmacother

Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.

Authors - Xufeng Tao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Gabriele (Verified Customer) (07-18-2025)

The antibody works very well both in WB (1:5000, 5% milk) and IF (1:200, 5% BSA) on HEK293T over-expressing human AMY2A 48h post-transfection vs not-treated control

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: WB: 1:5000 - IF: 1:200
  • Cell Tissue Type: HEK293T
Amylase Alpha Antibody Western Blot,Immunofluorescence validation (WB: 1:5000 - IF: 1:200 dilution) in HEK293T (Cat no:12540-1-AP)
FH

balawant (Verified Customer) (08-06-2022)

This antibody is working great and need 1:20000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20000
  • Cell Tissue Type: Intestinal tissue and colon cancer cell line
FH

Susmita (Verified Customer) (06-13-2022)

This is an excellent antibody for IHC and WB application

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 4000, IHC 1: 400
  • Cell Tissue Type: PC cell line PC tissue
FH

Iram (Verified Customer) (03-11-2022)

Very good antibody for western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colon cancer cells