AMZ1 Polyklonaler Antikörper

AMZ1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24484-1-AP

Synonyme

KIAA1950, EC:3.4.-.-, Archeobacterial metalloproteinase-like protein 1, Archaemetzincin-1, Archaemetzincin 1

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  WB analysis of mouse liver using 24484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24484-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24484-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24484-1-AP (AMZ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24484-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 24484-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24484-1-AP (AMZ1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 24484-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMZ1 antibody (24484-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24484-1-AP bindet in WB, IHC, IF/ICC, ELISA AMZ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMZ1 fusion protein Ag20248
• Vollständiger Name: archaelysin family metallopeptidase 1
• Berechnetes Molekulargewicht: 498 aa, 55 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 28 kDa
• GenBank-Zugangsnummer: BC146753
• Gene symbol: AMZ1
• Gene ID (NCBI): 155185
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMZ1, also named as KIAA1950, is a new human Zinc metalloproteases. It exhibits aminopeptidase activity against neurogranin in vitro. AMZ1 does not hydrolyze angiotensin-2. It is mainly detected in liver and heart. AMZ1 has some isoforms with MW 55 kDa, 33 kDa, 30 kDa and 28 kDa.