Angiogenin Polyklonaler Antikörper

Angiogenin Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18302-1-AP

Synonyme

ALS9, ANG, Angiogenin, HEL168, Ribonuclease 5, RNase 5, RNASE4, RNASE5

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 18302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human milk using 18302-1-AP

  human milk were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 18302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Milch-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

18302-1-AP bindet in WB, IHC, ELISA Angiogenin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: angiogenin, ribonuclease, RNase A family, 5
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC054880
• Gene symbol: ANG
• Gene ID (NCBI): 283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep Med; Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice; Authors - Jun-Ya Zhu
mouse	WB	Neurosci Bull; The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke; Authors - Yuxing Zhang
human	WB	FASEB J; MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.; Authors - Pei Zhang
human	WB	Front Oncol; Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.; Authors - Hui Tang
human	IHC	Front Med (Lausanne); Molecular and clinical characterization of ANG expression in gliomas and its association with tumor-related immune response; Authors - Jin Wang
rat	IHC	Life Sci; Sphingosine kinase 1/sphingosine 1-phosphate/sphingosine 1-phosphate receptor 1 pathway: A novel target of geniposide to inhibit angiogenesis.; Authors - Minghui Sun