Angiogenin Rekombinanter Antikörper

Angiogenin Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242738D1

Kat-Nr. : 85635-1-PBS

Synonyme

ANG, ANG 1, ANG I, ANG1, ANGI

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  WB analysis using 85635-1-RR (same clone as 85635-1-PBS)

  Untreated and Brefeldin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 85635-1-RR (ANG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85635-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85635-1-RR (same clone as 85635-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ANG antibody (85635-1-RR, Clone: 242738D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85635-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85635-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA Angiogenin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Angiogenin fusion protein Eg1930
• Vollständiger Name: angiogenin, ribonuclease, RNase A family, 5
• Berechnetes Molekulargewicht: 147 aa, 17 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC054880
• Gene symbol: Angiogenin
• Gene ID (NCBI): 283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.