Angiopoietin 2 Polyklonaler Antikörper

Angiopoietin 2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Rezensionen (2)

Kat-Nr. : 24613-1-AP

Synonyme

ANGPT2, AGPT 2, AGPT2, ANG 2, ANG2

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  WB analysis using 24613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24613-1-AP (Angiopoietin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 24613-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 24613-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Produktinformation

24613-1-AP bindet in WB, IHC, IF/ICC, ELISA Angiopoietin 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Angiopoietin 2 fusion protein Ag17938
• Vollständiger Name: angiopoietin 2
• Berechnetes Molekulargewicht: 496 aa, 57 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC126200
• Gene symbol: Angiopoietin 2
• Gene ID (NCBI): 285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin-2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin-2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin-2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin-2 is expressed only at sites of vascular remodeling. Angiopoietin-2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.

Publikationen

Species	Application	Title
human	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human	WB	J Med Chem; Discovery of Novel Small-Molecule Antiangiogenesis Agents to Treat Diabetic Retinopathy.; Authors - Donghwa Kim
human	WB	Int J Mol Sci; MiR-29a Curbs Hepatocellular Carcinoma Incidence via Targeting of HIF-1α and ANGPT2.; Authors - Ying-Hsien Huang
mouse	WB,IF	Exp Mol Med; Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization; Authors - Jung Min Ha
human	IHC	Front Oncol; MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis.; Authors - Hailin Li
human	IF	Biomed Pharmacother; Diosmetin inhibits tumor development and block tumor angiogenesis in skin cancer.; Authors - Jawun Choi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (07-25-2025)

The antibody detected several bands at dilution of 1:2000 incubated at room temperature for 2 hours followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A549

Angiopoietin 2 Antibody Western Blot validation (1:2000 dilution) in A549 (Cat no:24613-1-AP)

FH

Marine (Verified Customer) (07-01-2025)

It is not specific, it binds at different molecular weight on the ladder

• Applications: Western Blot
• Primary Antibody Dilution: 1/500 and 1/1000
• Cell Tissue Type: Tumoral cells, endothelial cells, mouse fibroblasts

Angiopoietin 2 Antibody Western Blot validation (1/500 and 1/1000 dilution) in Tumoral cells, endothelial cells, mouse fibroblasts (Cat no:24613-1-AP)