ANGPTL3 Rekombinanter Antikörper

ANGPTL3 Rekombinant Antikörper für WB, IHC, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250808F7

Kat-Nr. : 86193-1-PBS

Synonyme

ANG-5, Angiopoietin like protein 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3

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  WB analysis using 86193-1-RR (same clone as 86193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86193-1-RR (ANGPTL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02294-1

  Serum of sixteen individual healthy human donors was measured. The ANGPTL3 concentration of detected samples was determined to be 66.13 ng/mL with a range of 21.12-106.91 ng/mL.

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  Sandwich ELISA standard curve of MP02294-1

  Sandwich ELISA standard curve of MP02294-1, Human ANGPTL3 Recombinant Matched Antibody Pair - PBS only. 86193-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2573. 86193-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-100 ng/mL.

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  IHC staining of human hepatocellular cancer using 86193-1-RR (same clone as 86193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 86193-1-RR (same clone as 86193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86193-1-RR (same clone as 86193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86193-1-RR (same clone as 86193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 86193-1-RR (same clone as 86193-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using ANGPTL3 antibody (86193-1-RR, Clone: 250808F7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 86193-1-RR (same clone as 86193-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using ANGPTL3 antibody (86193-1-RR, Clone: 250808F7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86193-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02294-1

  The mean ANGPTL3 concentration was determined to be 14.8 ng/mL in human serum.

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  Cytometric bead array standard curve of MP02294-1

  Cytometric bead array standard curve of MP02294-1, ANGPTL3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86193-2-PBS. Detection antibody: 86193-1-PBS. Standard: Ag2573. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 86193-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86193-1-RR against Human ANGPTL3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86193-1-PBS bindet in WB, IHC, IF-Fro, Cytometric bead array, Sandwich ELISA, Indirect ELISA ANGPTL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ANGPTL3 fusion protein Ag2573
• Vollständiger Name: angiopoietin-like 3
• Berechnetes Molekulargewicht: 460 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC058287
• Gene symbol: ANGPTL3
• Gene ID (NCBI): 27329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.