ANGPTL3 Rekombinanter Antikörper

ANGPTL3 Rekombinant Antikörper für WB, IHC, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-Fro, ELISA

Konjugation

Unkonjugiert

CloneNo.

250808F7

Kat-Nr. : 86193-1-RR

Synonyme

ANG-5, Angiopoietin like protein 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3

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  WB analysis using 86193-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86193-1-RR (ANGPTL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocellular cancer using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocellular cancer using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86193-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86193-1-RR (ANGPTL3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 86193-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using ANGPTL3 antibody (86193-1-RR, Clone: 250808F7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of mouse kidney using 86193-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using ANGPTL3 antibody (86193-1-RR, Clone: 250808F7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86193-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86193-1-RR against Human ANGPTL3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A375-Zellen, HEK-293-Zellen, HuH-7-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: human hepatocellular cancer, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86193-1-RR bindet in WB, IHC, IF-Fro, ELISA ANGPTL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ANGPTL3 fusion protein Ag2573
• Vollständiger Name: angiopoietin-like 3
• Berechnetes Molekulargewicht: 460 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC058287
• Gene symbol: ANGPTL3
• Gene ID (NCBI): 27329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases.