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ANGPTL4 Polyklonaler Antikörper

ANGPTL4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Kat-Nr. : 18374-1-AP

Synonyme

ANGPTL2, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe, HEK-293-Zellen, Mausnierengewebe, Rattenherzgewebe
Erfolgreiche IPMausherzgewebe
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Herzgewebe, humanes Ösophaguskarzinomgewebe, humanes Eierstockgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCNIH/3T3-Zellen, HEK-293-Zellen, HT-29-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18374-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ANGPTL4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ANGPTL4 fusion protein Ag13237
Vollständiger Name angiopoietin-like 4
Berechnetes Molekulargewicht 45 kDa
Beobachtetes Molekulargewicht 45-50 kDa
GenBank-ZugangsnummerBC023647
Gene symbol ANGPTL4
Gene ID (NCBI) 51129
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ANGPTL4 antibody 18374-1-APProtokoll herunterladen
IHC protocol for ANGPTL4 antibody 18374-1-APProtokoll herunterladenl
IF protocol for ANGPTL4 antibody 18374-1-APProtokoll herunterladen
IP protocol for ANGPTL4 antibody 18374-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Nat Metab

Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota

Authors - Huating Li
mouseIHC

J Hazard Mater

Insight into perfluorooctanoic acid-induced impairment of mouse embryo implantation via single-cell RNA-seq

Authors - Xiaoqiang Sheng
humanIP

J Clin Invest

Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.

Authors - Akrit Sodhi
mouseWB

EMBO J

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang
mouseIF

J Pathol

ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2.

Authors - Elisa Gomez Perdiguero
humanIHC

Breast Cancer Res

An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer.

Authors - Misako Sato

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Niklas (Verified Customer) (11-28-2022)

bright and specific staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:350
  • Cell Tissue Type: human fibroblasts