ANK2 Polyklonaler Antikörper

ANK2 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31080-1-PBS

Synonyme

ANK 2, Ankyrin 2

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  WB analysis using 31080-1-AP (same clone as 31080-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31080-1-AP (ANK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31080-1-AP (ANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 31080-1-AP (same clone as 31080-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (31080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31080-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31080-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ANK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANK2 fusion protein Ag34642
• Vollständiger Name: ankyrin 2, neuronal
• Berechnetes Molekulargewicht: 434 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa
• GenBank-Zugangsnummer: NM_001148
• Gene symbol: ANK2
• Gene ID (NCBI): 287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)