ANK2 Rekombinanter Antikörper

ANK2 Rekombinant Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240982G5

Kat-Nr. : 83912-5-PBS

Synonyme

Ankyrin 2, ANK 2, 240982G5

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  IHC staining of mouse cerebellum using 83912-5-RR (same clone as 83912-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83912-5-RR (same clone as 83912-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 83912-5-RR (same clone as 83912-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83912-5-RR (same clone as 83912-5-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ank2 Recombinant Antibody (83912-5-RR, Clone:240982G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83912-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83912-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83912-5-RR against Human ANK2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83912-5-PBS bindet in IHC, IF-P, FC (Intra), ELISA ANK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ANK2 fusion protein Ag34642
• Vollständiger Name: ankyrin 2, neuronal
• Berechnetes Molekulargewicht: 434 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa
• GenBank-Zugangsnummer: NM_001148
• Gene symbol: ANK2
• Gene ID (NCBI): 287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)