ANK3 Polyklonaler Antikörper

ANK3 Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27766-1-AP

Synonyme

ANK 3, Ankyrin 3, ANKYRIN G, Ankyrin3, ANKYRING

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  IHC staining of mouse brain using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27766-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27766-1-AP (ANK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 27766-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27766-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27766-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ANK3 antibody (27766-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27766-1-AP bindet in IHC, IF-P, ELISA ANK3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANK3 fusion protein Ag26948
• Vollständiger Name: ankyrin 3, node of Ranvier (ankyrin G)
• Berechnetes Molekulargewicht: 480 kDa
• Beobachtetes Molekulargewicht: 204 kDa
• GenBank-Zugangsnummer: NM_001149
• Gene symbol: ANK3
• Gene ID (NCBI): 288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.