ANKIB1 Polyklonaler Antikörper

ANKIB1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30467-1-AP

Synonyme

ANKIB1, DKFZp434A0225, FLJ33123, KIAA1386

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Galerie der Validierungsdaten

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  WB analysis using 30467-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30467-1-AP (ANKIB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30467-1-AP

  IP result of anti-ANKIB1(IP:30467-1-AP, 4ug; Detection:30467-1-AP 1:4000) with HeLa cells lysate 1560 ug.

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  IHC staining of mouse kidney using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30467-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30467-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 30467-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Maushirngewebe, Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30467-1-AP bindet in WB, IP, IHC, IF, ELISA ANKIB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKIB1 fusion protein Ag33126
• Vollständiger Name: ankyrin repeat and IBR domain containing 1
• Berechnetes Molekulargewicht: 122 kDa
• Beobachtetes Molekulargewicht: 122 kDa
• GenBank-Zugangsnummer: NM_019004.1
• Gene symbol: ANKIB1
• Gene ID (NCBI): 54467
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ankyrin repeat and IBR domain-containing protein 1 (ANKIB1) is also named as KIAA1386, and belongs to the RBR family. ANKIB1 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. The common biomarkers found within the H and EC region of brain are ZNF621, SLC25A46, RAE1, and ANKIB1. Among these biomarkers, RAE1, ANKIB1, and SLC25A46 have been reported to be prominently involved in several neurodegenerative disorders (PMID:33878365). ANKIB1 is also found to be associated with Cerebral cavernous malformations (PMID:20798775).