ANKRD6 Polyklonaler Antikörper

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• KD/KO Validated

ANKRD6 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 24333-1-AP

Synonyme

Diversin, Ankyrin repeat domain-containing protein 6, ANKRD 6

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  WB analysis of HEK-293 using 24333-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 24333-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24333-1-AP

  IP result of anti-ANKRD6 (IP:24333-1-AP, 5ug; Detection:24333-1-AP 1:300) with HEK-293 cells lysate 920ug.

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  IHC staining of mouse ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Nierengewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

24333-1-AP bindet in WB, IHC, IP, ELISA ANKRD6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKRD6 fusion protein Ag19328
• Vollständiger Name: ankyrin repeat domain 6
• Berechnetes Molekulargewicht: 727 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC042173
• Gene symbol: ANKRD6
• Gene ID (NCBI): 22881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.

Publikationen

Species	Application	Title
human	IHC	Chin J Cancer Res; Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer.; Authors - Rui Bai
human	WB	Mol Carcinog; Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.; Authors - Kirthana Prabhakar ; KD Validated
human	IHC	Biomedicines; The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis; Authors - Claudia Alexandra Dumitru
rat	WB	Reprod Biol Endocrinol; Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin; Authors - Lingling Wang
human	WB	Endocrinology; The planar cell polarity (PCP) protein Fat 1 in Sertoli cell function; Authors - Tiao Bu