ANKRD6 Polyklonaler Antikörper

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• KD/KO Validated

ANKRD6 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24333-1-PBS

Synonyme

Diversin, Ankyrin repeat domain-containing protein 6, ANKRD 6

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  WB analysis of HEK-293 using 24333-1-AP (same clone as 24333-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 24333-1-AP (same clone as 24333-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 24333-1-AP (same clone as 24333-1-PBS)

  IP result of anti-ANKRD6 (IP:24333-1-AP, 5ug; Detection:24333-1-AP 1:300) with HEK-293 cells lysate 920ug. This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 24333-1-AP (same clone as 24333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 24333-1-AP (same clone as 24333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24333-1-AP (same clone as 24333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24333-1-AP (same clone as 24333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 24333-1-AP (same clone as 24333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 24333-1-AP (same clone as 24333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24333-1-PBS bindet in WB, IHC, IP, Indirect ELISA ANKRD6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKRD6 fusion protein Ag19328
• Vollständiger Name: ankyrin repeat domain 6
• Berechnetes Molekulargewicht: 727 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC042173
• Gene symbol: ANKRD6
• Gene ID (NCBI): 22881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.