ANKS1B Polyklonaler Antikörper

ANKS1B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24783-1-AP

Synonyme

AIDA, AIDA 1, Amyloid-beta protein intracellular domain-associated protein 1, ANKS2, Ankyrin repeat and sterile alpha motif domain-containing protein 1B

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  WB analysis of A549 using 24783-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24783-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24783-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24783-1-AP (ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24783-1-AP

  IP result of anti-ANKS1B (IP:24783-1-AP, 4ug; Detection:24783-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of mouse brain using 24783-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24783-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24783-1-AP (ANKS1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24783-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ANKS1B antibody (24783-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24783-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ANKS1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKS1B fusion protein Ag20454
• Vollständiger Name: ankyrin repeat and sterile alpha motif domain containing 1B
• Berechnetes Molekulargewicht: 1248 aa, 138 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC026313
• Gene symbol: ANKS1B
• Gene ID (NCBI): 56899
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANKS1B also named as AIDA or cajalin 2 is a amino acid protein, which contains 7 ANK repeats, 1 PID domain and 2 SAM domains. ANKS1B localizes in cytoplasm and is highly expressed in brain and testis. ANKS1B exists as many alternatively spliced isoforms. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing.

Publikationen

Species	Application	Title
human	WB	EBioMedicine; Juvenile CLN3 disease is a lysosomal cholesterol storage disorder: similarities with Niemann-Pick type C disease; Authors - Jacinda Chen