ANKS3 Polyklonaler Antikörper

ANKS3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 24058-1-AP

Synonyme

ANKS3, KIAA1977

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 24058-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 24058-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24058-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ANKS3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKS3 fusion protein Ag21252
• Vollständiger Name: ankyrin repeat and sterile alpha motif domain containing 3
• Berechnetes Molekulargewicht: 656 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa, 63 kDa
• GenBank-Zugangsnummer: BC050387
• Gene symbol: ANKS3
• Gene ID (NCBI): 124401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANKS3 (ankyrin repeat and sterile alpha motif domain containing 3) is a 656 amino acid protein that contain six ANK repeats and one SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. Two isoforms of ANKS3 exist due to alternative splicing events.This antibody may recognize both of them.

Publikationen

Species	Application	Title
human,mouse	WB,IF,IP,CoIP	PLoS Biol; Bicc1 ribonucleoprotein complexes specifying organ laterality are licensed by ANKS6-induced structural remodeling of associated ANKS3; Authors - Benjamin Rothé