ANLN Monoklonaler Antikörper

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• KD/KO Validated

ANLN Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1B4A8

Kat-Nr. : 66643-1-Ig

Synonyme

Anillin, Scraps, scra, anillin, actin binding protein, Actin binding protein anillin

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  WB analysis using 66643-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66643-1-Ig

  WB result of ANLN antibody (66643-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANLN transfected MCF-7 cells.

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  WB analysis of rat brain using 66643-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66643-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 66643-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANLN antibody (66643-1-Ig, Clone: 1B4A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, MCF-7-Zellen, Maushirngewebe, PC-12-Zellen, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66643-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA ANLN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ANLN fusion protein Ag19294
• Vollständiger Name: anillin, actin binding protein
• Berechnetes Molekulargewicht: 1124 aa, 124 kDa
• Beobachtetes Molekulargewicht: 110 kDa~120 kDa
• GenBank-Zugangsnummer: BC034692
• Gene symbol: ANLN
• Gene ID (NCBI): 54443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); ANLN Promotes the Proliferation and Migration of Gallbladder Cancer Cells via STRA6-Mediated Activation of PI3K/AKT Signaling; Authors - Xiang Zhu ; KD Validated
human	WB	Cell Cycle; CDK1-PLK1/SGOL2/ANLN pathway mediating abnormal cell division in cell cycle may be a critical process in hepatocellular carcinoma.; Authors - Ling Li
human	WB,IHC,CoIP	Mol Cell Biochem; PUS7 promotes the progression of pancreatic cancer by interacting ANLN to activate MYC pathway; Authors - Yubo Jiang
human	WB	Clin Exp Pharmacol Physiol; PMID: 32198770; Authors - Min Zhang
human	WB	Front Genet; ANLN and KDR Are Jointly Prognostic of Breast Cancer Survival and Can Be Modulated for Triple Negative Breast Cancer Control.; Authors - Xiaofeng Dai
human	WB	Pathol Res Pract; Vasculogenic mimicry-associated novel gene signature predicted prognosis and response to immunotherapy in lung adenocarcinoma; Authors - Lei Zhang