ANO2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ANO2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20647-1-AP

Synonyme

anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B

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  WB analysis using 20647-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20647-1-AP (ANO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20647-1-AP

  IP result of anti-ANO2 (IP:20647-1-AP, 4ug; Detection:20647-1-AP 1:300) with mouse brain tissue lysate 10000ug.

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  IHC staining of mouse eye using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat eye using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat eye using 20647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using ANO2 antibody (20647-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, humanes Mammakarzinomgewebe, Ratten-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Ratten-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

20647-1-AP bindet in WB, IHC, IF-P, IP, ELISA ANO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: anoctamin 2
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: NM_020373
• Gene symbol: ANO2
• Gene ID (NCBI): 57101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

Publikationen

Species	Application	Title
mouse,rat	IF	Nat Commun; Structural heterogeneity of the ion and lipid channel TMEM16F; Authors - Zhongjie Ye
mouse	IF	J Physiol; Functional expression of TMEM16A in taste bud cells.; Authors - Domenico M Guarascio ; KO Validated
mouse	WB	eNeuro; A role for STOML3 in olfactory sensory transduction.; Authors - Emilio Agostinelli
human	IF	Mol Cancer Res; Mechanisms of glioblastoma replication: Ca2+ flares and Cl- currents; Authors - Yunzhen Li