ANO2 Polyklonaler Antikörper

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• KD/KO Validated

ANO2 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20647-1-PBS

Synonyme

anoctamin 2, Anoctamin-2, C12orf3, TMEM16B, Transmembrane protein 16B

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  WB analysis using 20647-1-AP (same clone as 20647-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 20647-1-AP (ANO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 20647-1-AP (same clone as 20647-1-PBS)

  IP result of anti-ANO2 (IP:20647-1-AP, 4ug; Detection:20647-1-AP 1:300) with mouse brain tissue lysate 10000ug. This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20647-1-AP (same clone as 20647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 20647-1-AP (same clone as 20647-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using ANO2 antibody (20647-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20647-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20647-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA ANO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: anoctamin 2
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 114 kDa
• GenBank-Zugangsnummer: NM_020373
• Gene symbol: ANO2
• Gene ID (NCBI): 57101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.