ANO4 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

ANO4 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230308G1

Rezensionen (1)

Kat-Nr. : 82970-1-RR

Synonyme

Transmembrane protein 16D, TMEM16D, Anoctamin-4, anoctamin 4, 230308G1

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  WB analysis using 82970-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82970-1-RR (ANO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 82970-1-RR

  WB result of ANO4 antibody (82970-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANO4 transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Maushirngewebe, Maushodengewebe, PC-3-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82970-1-RR bindet in WB, ELISA ANO4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: anoctamin 4
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 100-111 kDa
• GenBank-Zugangsnummer: NM_178826
• Gene symbol: ANO4
• Gene ID (NCBI): 121601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANO4, also named as TMEM16D, belongs to the anoctamin family. ANO4 is primarily expressed in the central nervous system and certain endocrine glands, and it has been linked to a variety of neurological disorders (PMID: 36836529). The antibody recognize the isoform1 and isoform2 of ANO4.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vivienne (Verified Customer) (09-08-2025)

Lysates were prepared without cooking. 1x10^6 cells were incubated with RIPA buffer and sonified. After this BCA assay was performed for analysing the protein concentration. 50 µl plus 25 µl 3X Lämmli buffer + mercaptoethanol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 in 5 % BSA
• Cell Tissue Type: A374, SH-4

ANO4 Antibody Western Blot validation (1:1000 in 5 % BSA dilution) in A374, SH-4 (Cat no:82970-1-RR)