ANP32A Monoklonaler Antikörper

ANP32A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D8C5

Kat-Nr. : 67687-1-PBS

Synonyme

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

---

• 

  WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67687-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ANP32A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ANP32A fusion protein Ag8546
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Berechnetes Molekulargewicht: 249 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC007200
• Gene symbol: ANP32A
• Gene ID (NCBI): 8125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.