ANP32B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ANP32B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10843-1-AP

Synonyme

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10843-1-AP

  WB result of ANP32B antibody (10843-1-AP, 1:5000) with si-control and si-ANP32B transfected HEK293 cells.

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  WB analysis using 10843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10843-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10843-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 10843-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10843-1-AP

  IP Result of anti-ANP32B (IP:10843-1-AP, 3ug; Detection:10843-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human colon cancer using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10843-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10843-1-AP (ANP32B antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 10843-1-AP

  Immunofluorescent analysis of HeLa cells using 10843-1-AP (ANP32B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Produktinformation

10843-1-AP bindet in WB, IP, IHC, IF, ELISA ANP32B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANP32B fusion protein Ag1274
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC013003
• Gene symbol: ANP32B
• Gene ID (NCBI): 10541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Publikationen

Species	Application	Title
	WB	Sci Adv; The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals; Authors - Liuke Sun
mouse	WB	Proc Natl Acad Sci U S A; Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development.; Authors - Reilly Patrick T PT
human	WB	Carcinogenesis; Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.; Authors - Shen Shao-Ming SM ; KD Validated
human	WB	Blood Cancer J; PMID: 24413065; Authors - B C Valdez
human	WB,IHC	Cell Death Dis; ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.; Authors - S Yang ; KD Validated
human	WB	J Virol; ANP32 proteins are essential for influenza virus replication in human cells.; Authors - Ecco Staller ; KO Validated