ANP32B Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ANP32B Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

5A1F12

Kat-Nr. : 66160-1-Ig

Synonyme

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

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  WB analysis using 66160-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HEK-293 using 66160-1-Ig

  WB result of ANP32B antibody (66160-1-Ig, 1:5000) with si-control and si-ANP32B transfected HEK293 cells.

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  WB analysis of fetal human brain using 66160-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66160-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66160-1-Ig (ANP32B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66160-1-Ig (ANP32B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66160-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66160-1-Ig (ANP32B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66160-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32B antibody (66160-1-Ig, Clone: 5A1F12 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A431-Zellen, fetales humanes Hirngewebe, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:900-1:3600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

66160-1-Ig bindet in WB, IHC, IF/ICC, ELISA ANP32B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ANP32B fusion protein Ag1274
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC013003
• Gene symbol: ANP32B
• Gene ID (NCBI): 10541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Publikationen

Species	Application	Title
human	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	J Virol; ANP32 proteins are essential for influenza virus replication in human cells.; Authors - Ecco Staller ; KO Validated
mouse,rat	WB	Orphanet J Rare Dis; HDAC5 inhibition attenuates ventricular remodeling and cardiac dysfunction; Authors - Chenxi Zhu
human	WB	Nat Commun; Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction; Authors - Liuke Sun