ANP32CP Polyklonaler Antikörper

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• KD/KO Validated

ANP32CP Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31298-1-AP

Synonyme

ANP32C, PP32R1, Tumorigenic protein pp32r1

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  WB analysis of DU 145 using 31298-1-AP

  WB result of ANP32CP antibody (31298-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANP32CP transfected DU 145 cells.

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  WB analysis using 31298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31298-1-AP (ANP32CP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 31298-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ANP32CP antibody (31298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, LNCaP-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31298-1-AP bindet in WB, ELISA ANP32CP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANP32CP fusion protein Ag35293
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: NM_012403.1
• Gene symbol: ANP32C/PP32R1
• Gene ID (NCBI): 23520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.