ANP32CP Rekombinanter Antikörper

ANP32CP Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241562D12

Kat-Nr. : 84553-1-RR

Synonyme

ANP32C, Tumorigenic protein pp32r1, PP32R1, 241562D12

---

• 

  WB analysis using 84553-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84553-1-RR (ANP32CP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of LNCaP using 84553-1-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using ANP32CP antibody (84553-1-RR, Clone: 241562D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84553-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84553-1-RR against Human ANP32CP were performed. The affinity constant is 7.76 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, BxPC-3-Zellen, DU 145-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84553-1-RR bindet in WB, IF/ICC, ELISA ANP32CP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ANP32CP fusion protein Ag35293
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: NM_012403.1
• Gene symbol: ANP32C/PP32R1
• Gene ID (NCBI): 23520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.