ANP32E Polyklonaler Antikörper

ANP32E Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31989-1-PBS

Synonyme

Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E, Acidic leucine-rich nuclear phosphoprotein 32 family member E, Acidic Nuclear Phosphoprotein 32 Family Member E, LANPL, LANP-L

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  WB analysis using 31989-1-AP (same clone as 31989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31989-1-AP (ANP32E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 31989-1-AP (same clone as 31989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 31989-1-AP (same clone as 31989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 31989-1-AP (same clone as 31989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ANP32E antibody (31989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31989-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA ANP32E und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANP32E fusion protein Ag33772
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC003380
• Gene symbol: ANP32E
• Gene ID (NCBI): 81611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acidic nuclear phosphoprotein 32 family member E (ANP32E) is a histone chaperone that removes H2A.Z from chromatin. ANP32E is implicated in many cellular processes, including chromatin modification and remodeling, gene regulation, protein phosphorylation, and regulation of intracellular trafficking, and contributes to early development and cancer metastasis in mammals. In addition, ANP32E is involved in tumor development and is highly expressed in pancreatic and thyroid cancer cells, and promotes tumorigenesis in gastric cancer (GC) cells by inducing NUF2 expression.