CD13 Polyklonaler Antikörper

CD13 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14553-1-AP

Synonyme

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

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Galerie der Validierungsdaten

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  WB analysis using 14553-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14553-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14553-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14553-1-AP (CD13 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 14553-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 14553-1-AP (CD13 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 14553-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug CD13 antibody (14553-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, humanes Nierengewebe, Mauslebergewebe, Mausnierengewebe, PC-3-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

14553-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CD13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Hund, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD13 fusion protein Ag5976
• Vollständiger Name: alanyl (membrane) aminopeptidase
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC058928
• Gene symbol: ANPEP
• Gene ID (NCBI): 290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
human		Anal Chem; Rapid Screening of Peptide Probes through in situ Single Bead Sequencing Microarray.; Authors - Weizhi Wang
human	WB,IF	Neuropathol Appl Neurobiol; Brain mural cell loss in the parietal cortex in Alzheimer's disease correlates with cognitive decline and TDP-43 pathology.; Authors - P Bourassa
human	WB	Front Oncol; YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma.; Authors - Qiang Xie
rat	WB	PLoS Negl Trop Dis; Proteomic Analysis of Urine Exosomes Reveals Renal Tubule Response to Leptospiral Colonization in Experimentally Infected Rats.; Authors - Satish P RamachandraRao
mouse	IF	Exp Cell Res; siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.; Authors - Shion Osana