ANT1/2 Polyklonaler Antikörper

ANT1/2 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

Konjugation

Unkonjugiert

Kat-Nr. : 15997-1-PBS

Synonyme

SLC25A4, AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT

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  WB analysis of human heart using 15997-1-AP (same clone as 15997-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15997-1-AP (same clone as 15997-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15997-1-AP (same clone as 15997-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 15997-1-AP (same clone as 15997-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 15997-1-AP (same clone as 15997-1-PBS)

  IP result of anti-ANT1/2 (IP:15997-1-AP, 4ug; Detection:15997-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 15997-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15997-1-PBS bindet in ANT1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANT1/2 fusion protein Ag7787
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC008664
• Gene symbol: SLC25A4
• Gene ID (NCBI): 291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.