ANTXR1 Polyklonaler Antikörper

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• KD/KO Validated

ANTXR1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15091-1-PBS

Synonyme

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  WB analysis of mouse testis using 15091-1-AP (same clone as 15091-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15091-1-AP (same clone as 15091-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-13 using 15091-1-AP (same clone as 15091-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 15091-1-AP (same clone as 15091-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15091-1-AP (same clone as 15091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15091-1-AP (same clone as 15091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15091-1-AP (same clone as 15091-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15091-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ANTXR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANTXR1 fusion protein Ag4626
• Vollständiger Name: anthrax toxin receptor 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 85 kDa, 80 kDa
• GenBank-Zugangsnummer: BC012074
• Gene symbol: ANTXR1
• Gene ID (NCBI): 84168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).