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ANUBL1 Polyklonaler Antikörper

ANUBL1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17478-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ANUBL1 Polyclonal antibody (17478-1-AP)

    WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ANUBL1 Polyclonal antibody (17478-1-AP)

    WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANUBL1 Polyclonal antibody (17478-1-AP)

    IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ANUBL1 Polyclonal antibody (17478-1-AP)

    IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17478-1-PBS bindet in WB, IHC, Indirect ELISA ANUBL1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ANUBL1 fusion protein Ag11505
Vollständiger Name AN1, ubiquitin-like, homolog (Xenopus laevis)
Berechnetes Molekulargewicht 653 aa, 72 kDa
Beobachtetes Molekulargewicht70-80 kDa
GenBank-ZugangsnummerBC048968
Gene symbol ANUBL1
Gene ID (NCBI) 93550
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).