Annexin A2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Annexin A2 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

CloneNo.

3H1B11

Kat-Nr. : 60051-1-Ig

Synonyme

ANXA2, 3H1B11, Annexin 2, Annexin II, Annexin-2

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  WB analysis using 60051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 60051-1-Ig

  WB result of Annexin A2 antibody (60051-1-Ig, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells.

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  WB analysis of THP-1 using 60051-1-Ig

  WB result of Annexin A2 antibody (60051-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells.

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  IP experiment of HeLa using 60051-1-Ig

  IP result of anti-Annexin A2 (IP:60051-1-Ig, 4ug; Detection:60051-1-Ig 1:1500) with HeLa cells lysate 2400ug.

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  IHC staining of human breast cancer using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60051-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50.

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  IHC staining of human gliomas using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60051-1-Ig (ANXA2 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60051-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of T-47D using 60051-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 60051-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (60051-1-Ig, Clone: 3H1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HeLa using 60051-1-Ig

  Immunofluorescent analysis of Hela cells, using ANXA2 antibody 66051-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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  IF Staining of MCF-7 using 60051-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using ANXA2 antibody 60051-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  IF Staining of HeLa using 60051-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, 4T1-Zellen, A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, THP-1-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: T-47D-Zellen, HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

60051-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Annexin A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin A2 fusion protein Ag1777
• Vollständiger Name: annexin A2
• Berechnetes Molekulargewicht: 339 aa, 39 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC016774
• Gene symbol: Annexin A2
• Gene ID (NCBI): 302
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Publikationen

Species	Application	Title
mouse	WB,CoIP	Cell; Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice; Authors - Kaili Fu
human	IP	EBioMedicine; Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.; Authors - Xiao Li
		Infect Immun; The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation.; Authors - Xiaowei Wang
mouse	WB	Cell Mol Life Sci; Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation; Authors - Yunjian Dai
mouse,human	WB,IP,IF	iScience; Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors; Authors - Megan Sheridan ; KD Validated
human	WB,IF	Cell Signal; The novel DNA methylation marker FIBIN suppresses non-small cell lung cancer metastasis by negatively regulating ANXA2; Authors - Mingyu Peng