Annexin A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Annexin A3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11804-1-AP

Synonyme

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III

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Galerie der Validierungsdaten

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  WB analysis using 11804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11804-1-AP (Annexin A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11804-1-AP (Annexin A3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11804-1-AP

  IP Result of anti-Annexin A3 (IP:11804-1-AP, 4ug; Detection:11804-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11804-1-AP (Annexin A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

11804-1-AP bindet in WB, IP, IHC, IF, ELISA Annexin A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Annexin A3 fusion protein Ag2381
• Vollständiger Name: annexin A3
• Berechnetes Molekulargewicht: 322 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000871
• Gene symbol: ANXA3
• Gene ID (NCBI): 306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Upregulated of ANXA3, SORL1, and Neutrophils May Be Key Factors in the Progressionof Ankylosing Spondylitis.; Authors - Jie Jiang
rat	IF	iScience; Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury; Authors - Yu Zhang
mouse	IF	Sci Rep; Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.; Authors - R Sartaj
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao
mouse	WB,IHC	J Proteomics; Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.; Authors - Fang Jiao
rat	WB	J Agric Food Chem; Effect of High-Fat Diet on Hepatic Proteomics of Hamsters.; Authors - Chen-Chung Liao