Annexin A4 Polyklonaler Antikörper

Annexin A4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 10087-1-AP

Synonyme

ANXA4, ANX4, Annexin-4, Annexin IV, Annexin 4

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• 

  WB analysis using 10087-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10087-1-AP (Annexin IV antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10087-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10087-1-AP (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 10087-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10087-1-AP (Annexin A4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

10087-1-AP bindet in WB, IHC, ELISA Annexin A4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Annexin A4 fusion protein Ag0130
• Vollständiger Name: annexin A4
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC000182
• Gene symbol: Annexin A4
• Gene ID (NCBI): 307
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.; Authors - Rogers Ronan S RS
human	WB	Aging (Albany NY); Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.; Authors - Ya Peng
mouse	WB	J Immunol; Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.; Authors - Kulik Liudmila L
human	WB	Mol Cell Proteomics; Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.; Authors - Hussein Kaddour
human	WB,IHC	Sci Rep; Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification.; Authors - Kewu Jiang
human	WB	PLoS One; Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.; Authors - Qi Ying

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuan (Verified Customer) (09-30-2019)

The antibody is working very well on HeLa cells.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

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