Annexin V Rekombinanter Antikörper

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• KD/KO Validated

Annexin V Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240621D1

Kat-Nr. : 83929-1-RR

Synonyme

Annexin A5, ANXA5, 240621D1, Anchorin CII, Annexin 5

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  WB analysis using 83929-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83929-1-RR (ANXA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 83929-1-RR

  WB result of Annexin V antibody (83929-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells.

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  Affinity and Kinetic Characterization of 83929-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83929-1-RR against Human ANXA5 were performed. The affinity constant is 13.4 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83929-1-RR bindet in WB, ELISA Annexin V und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Annexin V fusion protein Ag0741
• Vollständiger Name: annexin A5
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC001429
• Gene symbol: Annexin V
• Gene ID (NCBI): 308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.