AOAH Polyklonaler Antikörper

AOAH Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12911-1-AP

Synonyme

Acyloxyacyl hydrolase, Acyloxyacyl hydrolase large subunit, Acyloxyacyl hydrolase small subunit, EC:3.1.1.77

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  WB analysis of mouse colon using 12911-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12911-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12911-1-AP (AOAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12911-1-AP (AOAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

12911-1-AP bindet in WB, IHC, IP, ELISA AOAH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AOAH fusion protein Ag3892
• Vollständiger Name: acyloxyacyl hydrolase (neutrophil)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC025698
• Gene symbol: AOAH
• Gene ID (NCBI): 313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acyloxyacyl hydrolase (AOAH) is a highly conserved lipase responsible for hydrolyzing the secondary fatty acyl chains of gram-negative bacterial lipopolysaccharides (LPS), resulting in LPS detoxification and attenuation of host inflammation (PMID: 1577781, PMID: 35980963). AOAH has also been shown to have PLA2 function (PMID: 1577781). AOAH was highly expressed at the protein level in lesional psoriatic skin compared to healthy controls (PMID: 34913478). AOAH has been described to be differentially proteolytically processed into both a secreted and cytosolic form in phagocytic cells (PMID: 19755182). Expression of AOAH is primarily attributed to phagocytic cells such as neutrophils, macrophages, DC, and also NK cells (PMID: 33454018).

Publikationen

Species	Application	Title
	WB,IP	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han