VAP1/AOC3 Polyklonaler Antikörper

VAP1/AOC3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 14365-1-AP

Synonyme

AOC3, VAP1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase

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  WB analysis using 14365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 14365-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Maus-Fettgewebe, Maus-Magengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Lungenkarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

14365-1-AP bindet in WB, IHC, ELISA VAP1/AOC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAP1/AOC3 fusion protein Ag5683
• Vollständiger Name: amine oxidase, copper containing 3 (vascular adhesion protein 1)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC050549
• Gene symbol: AOC3
• Gene ID (NCBI): 8639
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Publikationen

Species	Application	Title
human	IHC	J Cell Mol Med; Single-cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences.; Authors - Fengchan Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (10-21-2020)

Right size band and Clean background, but with relatively weak signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver lysate

VAP1/AOC3 Antibody Western Blot validation (1:1000 dilution) in mouse liver lysate (Cat no:14365-1-AP)