VAP1/AOC3 Monoklonaler Antikörper

VAP1/AOC3 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2A6G3

Kat-Nr. : 66834-1-Ig

Synonyme

AOC3, VAP1, 2A6G3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3

---

• 

  WB analysis of human ileum using 66834-1-Ig

  human ileum tissue were subjected to SDS PAGE followed by western blot with 66834-1-Ig (AOC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66834-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66834-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Ileumgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

66834-1-Ig bindet in WB, IHC, IF-P, ELISA VAP1/AOC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VAP1/AOC3 fusion protein Ag5908
• Vollständiger Name: amine oxidase, copper containing 3 (vascular adhesion protein 1)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC050549
• Gene symbol: AOC3
• Gene ID (NCBI): 8639
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Publikationen

Species	Application	Title
human	IHC	Front Immunol; A novel immune cell signature for predicting osteosarcoma prognosis and guiding therapy; Authors - Runsang Pan
human	IHC	Commun Biol; Crosstalk of cell death pathways unveils an autophagy-related gene AOC3 as a critical prognostic marker in colorectal cancer; Authors - Hui Xu
human	WB	J Transl Med; Comprehensive analysis of the metabolomics and transcriptomics uncovers the dysregulated network and potential biomarkers of Triple Negative Breast Cancer; Authors - Sisi Gong