Aldehyde oxidase Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Aldehyde oxidase Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 19495-1-AP

Synonyme

Aldehyde oxidase, aldehyde oxidase 1, AO, AOH1, AOX1

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Galerie der Validierungsdaten

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  WB analysis using 19495-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19495-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19495-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 19495-1-AP

  IP Result of anti-Aldehyde oxidase (IP:19495-1-AP, 4ug; Detection:19495-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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  IHC staining of human hepatocirrhosis using 19495-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 19495-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19495-1-AP

  Immunofluorescent analysis of HepG2 cells, using AOX1 antibody 19495-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mauslungengewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

19495-1-AP bindet in WB, IP, IHC, IF, ELISA Aldehyde oxidase und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: aldehyde oxidase 1
• Berechnetes Molekulargewicht: 148 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: NM_001159
• Gene symbol: AOX1
• Gene ID (NCBI): 316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	EMBO Rep; Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.; Authors - Laura M Zidek
human	WB,IHC	Oncogene; Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.; Authors - Venkatrao Vantaku ; KD Validated
human	IF	Am J Obstet Gynecol; Evidence that fetal death is associated with placental aging.; Authors - Kaushik Maiti
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
human	WB	Front Immunol; Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.; Authors - Shun-Li Peng
human	WB	Cell Signal; Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway; Authors - Ziheng Yuan