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AP1M1 Polyklonaler Antikörper

AP1M1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12112-1-PBS

Synonyme

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  • Western Blot (WB) analysis of Hacat cells using AP1M1 Polyclonal antibody (12112-1-AP)

    WB analysis of Hacat cells using 12112-1-AP (same clone as 12112-1-PBS)

    WB resut of AP1M1 antibody (12112-1-AP, 1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using AP1M1 Polyclonal antibody (12112-1-AP)

    WB analysis of human brain using 12112-1-AP (same clone as 12112-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using AP1M1 Polyclonal antibody (12112-1-AP)

    WB analysis of SH-SY5Y using 12112-1-AP (same clone as 12112-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using AP1M1 Polyclonal antibody (12112-1-AP)

    WB analysis of rat brain using 12112-1-AP (same clone as 12112-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using AP1M1 Polyclonal antibody (12112-1-AP)

    IP experiment of SH-SY5Y using 12112-1-AP (same clone as 12112-1-PBS)

    IP result of anti-AP1M1 (IP:12112-1-AP, 4ug; Detection:12112-1-AP 1:500) with SH-SY5Y cells lysate 2500ug. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12112-1-PBS bindet in WB, IP, Indirect ELISA AP1M1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AP1M1 fusion protein Ag2757
Vollständiger Name adaptor-related protein complex 1, mu 1 subunit
Berechnetes Molekulargewicht 423 aa, 49 kDa
Beobachtetes Molekulargewicht 49 kDa
GenBank-ZugangsnummerBC017469
Gene symbol AP1M1
Gene ID (NCBI) 8907
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.