AP1M1 Monoklonaler Antikörper

AP1M1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9A11

Kat-Nr. : 68726-1-PBS

Synonyme

AP-1 complex subunit mu-1, AP mu chain family member mu1A, AP 1 complex subunit mu 1, Adaptor-related protein complex 1 subunit mu-1, Adaptor protein complex AP-1 subunit mu-1

---

• 

  WB analysis using 68726-1-Ig (same clone as 68726-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68726-1-Ig (same clone as 68726-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 68726-1-Ig (same clone as 68726-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 68726-1-Ig (same clone as 68726-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 68726-1-Ig (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68726-1-Ig (same clone as 68726-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AP1M1 antibody (68726-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68726-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68726-1-PBS bindet in WB, IF/ICC, Indirect ELISA AP1M1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AP1M1 fusion protein Ag25303
• Vollständiger Name: adaptor-related protein complex 1, mu 1 subunit
• Berechnetes Molekulargewicht: 423 aa, 49 kDa
• Beobachtetes Molekulargewicht: 47-49 kDa
• GenBank-Zugangsnummer: BC017469
• Gene symbol: AP1M1
• Gene ID (NCBI): 8907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.