AP1M2 Polyklonaler Antikörper

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• KD/KO Validated

AP1M2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10618-1-PBS

Synonyme

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  WB analysis of Hacat cells using 10618-1-AP (same clone as 10618-1-PBS)

  WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 10618-1-AP (same clone as 10618-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 10618-1-AP (same clone as 10618-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 10618-1-AP (same clone as 10618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 10618-1-AP (same clone as 10618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10618-1-AP (same clone as 10618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10618-1-AP (same clone as 10618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10618-1-PBS bindet in WB, IHC, Indirect ELISA AP1M2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP1M2 fusion protein Ag0961
• Vollständiger Name: adaptor-related protein complex 1, mu 2 subunit
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC005021
• Gene symbol: AP1M2
• Gene ID (NCBI): 10053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.